Pollination following grafting introduces efficiently Ocimum basilicum L. genes into Nicotiana tabacum L.
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Coproporphyrinogenase in tobacco (Nicotiana tabacum L.).
1. Coproporphyrinogenase was extracted and purified from tobacco (Nicotiana tabacum L.). Enzyme activity was mainly located in mitochondria rather than in chloroplasts. The enzyme was purified by differential centrifugation, ammonium sulphate fractionation, calcium phosphate gel adsorption and dialysis. A 69-fold final purification was obtained. 2. An apparent K(m) value of 3.6x10(-5)m was foun...
متن کاملHepatoprotective triterpenes from hairy root cultures of Ocimum basilicum L.
Six triterpene acids identified as betulinic, oleanolic, ursolic, 3-epimaslinic, alphitolic and euscaphic acids have been isolated from a dichloromethane extract of hairy root cultures of Ocimum basilicum L. (Lamiaceae). These cultures were obtained by genetic transformation using Agrobacterium rhizogenes. The extract as well as the isolated compounds were evaluated for their hepatoprotective a...
متن کاملChicoric acid found in basil (Ocimum basilicum L.) leaves
This is the first report to identify the presence of chicoric acid (cichoric acid; also known as dicaffeoyltartaric acid, which is a caffeic acid derivatizedwith tartaric acid) in basil leaves. Rosmarinic acid, chicoric acid and caftaric acid (in the order of most abundant to least; all derivatives of caffeic acid) were identified in fresh basil leaves. Rosmarinic acid was themain phenolic comp...
متن کاملAcylated Steryl Glycoside Synthesis in Seedlings of Nicotiana tabacum L.
In tobacco seedlings (Nicotiana tabacum L.), glucose from supplied uridine diphosphate-[U-(14)C]glucose was first incorporated into steryl glycosides and later into acylated steryl glycosides. However, when [(14)C]cholesterol was used as substrate, the acylated steryl glycosides became labeled earlier than the steryl glycosides. With [(14)C]cholesteryl glucoside as substrate, most of the radioa...
متن کاملSteryl Glycoside Formation in Seedlings of Nicotiana tabacum L.
Particulate enzyme preparations from tobacco seedlings (Nicotiana tabacum L.) were used in the synthesis of steryl glycoside. The data obtained by measuring cholesterol-4-(14)C incorporation generally agree with results obtained with UDP-glucose-(14)C. The in vitro reaction was linear for the first 10 minutes and had a pH optimum of 7.0 to 7.4. Addition of ATP activated while UDP-glucose inhibi...
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ژورنال
عنوان ژورنال: Spanish Journal of Agricultural Research
سال: 2013
ISSN: 2171-9292,1695-971X
DOI: 10.5424/sjar/2013114-3940